Postgraduate Course: Quantitating drug binding (PGBI11038)
Course Outline
| School |
School of Biological Sciences |
College |
College of Science and Engineering |
| Course type |
Standard |
Availability |
Not available to visiting students |
| Credit level (Normal year taken) |
SCQF Level 11 (Postgraduate) |
Credits |
10 |
| Home subject area |
Postgraduate |
Other subject area |
None |
| Course website |
None |
|
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| Course description |
In three practical sessions, students individually will determine the binding constant of an agonist to rat-brain muscarinic acetylcholine receptor using a filter assay to measure bound receptor. Further sessions will derive the binding constants and total number of receptors from the data, including use of non-linear least squares fit to the hyperbola describing binding. This analysis will be used to determine variances for the parameters fitted and will lead to a critical examination of the design of the experiment. Other techniques for determining binding constants will be considered, with an emphasis on the appropriate windows in which they can be applied and on potential artefacts and pitfalls. |
Course Delivery Information
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| Delivery period: 2010/11 Semester 1, Not available to visiting students (SS1)
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WebCT enabled: Yes |
Quota: None |
| Location |
Activity |
Description |
Weeks |
Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
| King's Buildings | Lecture | | 1-11 | 09:00 - 12:00 | | | | |
| First Class |
First class information not currently available |
Summary of Intended Learning Outcomes
Overall: - to have a deeper knowledge and critical appreciation of how binding constants for drug molecules to their targets are determined.
Specifically:
- be competent to carry out the experimental manipulations to determine a binding constant of a drug to its target using a radioactive filter assay;
- be able to work safely with radioactivity;
- understand how to derive a binding constant of a drug to its target and the stoichiometry;
- use a spreadsheet to carry out calculations;
- apply non-linear least squares methods to fit equations and to derive the variances in the parameters fitted;
- appreciate how best to design an experiment so that the parameters are well determined;
- acquire working knowledge of a number of techniques to determine drug binding to their targets;
- design and populate a poster explaining a specific technique used to measure drug binding; and
- show, through a blog, that you can reflect at a deep level on the material in the course and provide evidence that you have achieved the learning outcomes. |
Assessment Information
Extended Practical report 50 %
Poster on a specific technique 30 % (including feedback on other?s pages)
Blog 20 %
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Special Arrangements
| Not entered |
Contacts
| Course organiser |
Dr Paul Mclaughlin
Tel: (0131 6)50 7060
Email: paul.mclaughlin@ed.ac.uk |
Course secretary |
Miss Vicky Mactaggart
Tel: (0131 6)51 7052
Email: Vicky.Mactaggart@ed.ac.uk |
|
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1 September 2010 6:24 am
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