Postgraduate Course: Detailed Characterisation of Drug or Ligand Interactions Using Surface Plasmon Resonance (SPR) (BILG11005)
Course Outline
| School | School of Biological Sciences |
College | College of Science and Engineering |
| Course type | Standard |
Availability | Not available to visiting students |
| Credit level (Normal year taken) | SCQF Level 11 (Postgraduate) |
Credits | 10 |
| Home subject area | Postgraduate |
Other subject area | None |
| Course website |
None |
Taught in Gaelic? | No |
| Course description | This course will provide both theoretical and some hands on-experience of the practical use of Surface Plasmon Resonance to characterise biophysical interactions, and will show how the affinity, kinetic and thermodynamic data generated from such experiments can be used to enrich and accelerate the progress of accurate SARs in the development of drugs and therapeutics. The data handling and processing of affinity/kinetic and thermodynamic data will be applicable to other biophysical characterisation techniques. |
Entry Requirements (not applicable to Visiting Students)
| Pre-requisites |
|
Co-requisites | |
| Prohibited Combinations | |
Other requirements | None |
| Additional Costs | 0 |
Course Delivery Information
|
| Delivery period: 2013/14 Semester 2, Not available to visiting students (SS1)
|
Learn enabled: Yes |
Quota: None |
|
Web Timetable |
Web Timetable |
| Course Start Date |
13/01/2014 |
| Breakdown of Learning and Teaching activities (Further Info) |
Total Hours:
100
(
Lecture Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
68 )
|
| Additional Notes |
|
| Breakdown of Assessment Methods (Further Info) |
Written Exam
0 %,
Coursework
100 %,
Practical Exam
0 %
|
| No Exam Information |
Summary of Intended Learning Outcomes
| On successful completion of the course, students should be able to demonstrate an understanding of the technologies that underpin SPR instruments and experiments. They should also have demonstrated the ability to successfully generate and analyse an example dataset of affinity, kinetic and thermodynamic data. They should be able to discuss their results in a relation to the basic SARs of a model example protein:small molecule system. |
Assessment Information
In Course Assessment - 100%
An ICA consisting of a data analysis of an example dataset of affinity, kinetic and thermodynamic data and generation of report describing development of further experiments/considerations for development of the theoretical drug/therapeutic based on the SARs generated/supplied. |
Additional Information
| Academic description |
Surface plasmon resonance (SPR) is routinely exploited in the kinetic analysis of biomolecular interactions and small-molecule drug-discovery/hit-validation studies. SPR instruments uniquely allow the measurement of kinetic and thermodynamic data specifically associated with complex formation and dissociation. Such SPR data can be used to "thermodynamic profile" interactions enhancing the correlation of solution binding measurements with structural features and the assignment of proportional energetic contributions to individual functional groups, the basis of the whole-structure-based design approach to engineered therapeutics and drug design. This course will provide both theoretical and some hands on-experience of the practical use of SPR to characterise biophysical interactions, and will show how the affinity, kinetic and thermodynamic data generated from such experiments can be used to enrich and accelerate the progress of accurate SARs in the development of drugs and therapeutics. The data handling and processing of affinity/kinetic and thermodynamic data will be applicable to other biophysical characterisation techniques. |
| Syllabus |
Theoretical background of SPR.
Practical instrumental and experimental considerations for surface and data generation with small molecules and fragments.
Thermodynamic profiling
Data analysis - kinetic, affinity and thermodynamic.
Use of SPR data for small molecule:protein SARs.
|
| Transferable skills |
Understanding of Surface plasmon resonance and its uses |
| Reading list |
N/A |
| Study Abroad |
N/A |
| Study Pattern |
Study will be a mixture of Lectures, references provides as reading Material and practical sessions involving the computation analysis of example data sets |
| Keywords | SPR |
Contacts
| Course organiser | Dr Martin Wear
Tel:
Email: Martin.Wear@ed.ac.uk |
Course secretary | Miss Vicky Mactaggart
Tel: (0131 6)51 7052
Email: Vicky.Mactaggart@ed.ac.uk |
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