Postgraduate Course: Practical Skills in Biochemistry B (PGBI11099)
Course Outline
| School | School of Biological Sciences |
College | College of Science and Engineering |
| Credit level (Normal year taken) | SCQF Level 11 (Postgraduate) |
Availability | Not available to visiting students |
| SCQF Credits | 10 |
ECTS Credits | 5 |
| Summary | The programme provides thorough training and hands-on experience in enhanced practical skills required for employment as a biochemical scientist. The course will cover a range of techniques from recombinant protein overexpression and purification, followed by activity assays and biophysical characterisation. |
| Course description |
Not entered
|
Entry Requirements (not applicable to Visiting Students)
| Pre-requisites |
|
Co-requisites | |
| Prohibited Combinations | |
Other requirements | None |
Course Delivery Information
|
| Academic year 2014/15, Not available to visiting students (SS1)
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Quota: None |
| Course Start |
Semester 2 |
Timetable |
Timetable |
| Learning and Teaching activities (Further Info) |
Total Hours:
100
(
Lecture Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
68 )
|
| Assessment (Further Info) |
Written Exam
0 %,
Coursework
100 %,
Practical Exam
0 %
|
| Additional Information (Assessment) |
Two written assessments:
Design experimental strategy fo biophysical and activity characterisation of purified protein (40%)
Experimental write-up, in the form, of a paper of practicals in 1-8 (60%)
|
| Feedback |
Not entered |
| No Exam Information |
Learning Outcomes
On completion of this course, students will be able to:
Extract and purify recombinant protein from E. coli by affinity chromatography
Describe how and why differences in experimental conditions or available equipment will influence the experimental strategy and outcome
Identify the applicable methods required and design an appropriate strategy for biophysical characterisation of a biomolecule
Apply, or be able to explain the advantages and limitations of, a range of biophysical characterisation methods
Design, implement and analyse a straightforward enzyme assay
Describe the advantages and limitations of different methods for determining binding affinities
Have confidence in learning the operation of advanced instrumentation
|
Additional Information
| Graduate Attributes and Skills |
Confidence in laboratory environment
Report writing
Data management
Appreciation of health and safety responsibilities
Time management
Organisation
Group work
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| Keywords | BioChemB |
Contacts
| Course organiser | Dr Janice Bramham
Tel: (0131 6)50 4704
Email: janice.bramham@ed.ac.uk |
Course secretary | Ms Andrea Nichol
Tel: (0131 6)50 5538
Email: Andrea.Nichol@ed.ac.uk |
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