Postgraduate Course: Detailed Characterisation of Drug or Ligand Interactions Using Surface Plasmon Resonance (SPR) (BILG11005)
|School||School of Biological Sciences
||College||College of Science and Engineering
|Credit level (Normal year taken)||SCQF Level 11 (Postgraduate)
||Availability||Not available to visiting students
|Summary||This course will discuss the practical use of Surface Plasmon Resonance to characterise biophysical interactions, and will show how the affinity, kinetic and thermodynamic data generated from such experiments can be used to enrich and accelerate the progress of accurate SARs in the development of drugs and therapeutics. The data handling and processing of affinity/kinetic and thermodynamic data will be applicable to other biophysical characterisation techniques.
Theoretical background of SPR.
Practical instrumental and experimental considerations for surface and data generation with small molecules and fragments.
Data analysis - kinetic, affinity and thermodynamic.
Use of SPR data for small molecule:protein SARs.
Surface plasmon resonance (SPR) is routinely exploited in the kinetic analysis of biomolecular interactions and small-molecule drug-discovery/hit-validation studies. SPR instruments uniquely allow the measurement of kinetic and thermodynamic data specifically associated with complex formation and dissociation. Such SPR data can be used to "thermodynamic profile" interactions enhancing the correlation of solution binding measurements with structural features and the assignment of proportional energetic contributions to individual functional groups, the basis of the whole-structure-based design approach to engineered therapeutics and drug design. This course will provide both theoretical and some hands on-experience of the practical use of SPR to characterise biophysical interactions, and will show how the affinity, kinetic and thermodynamic data generated from such experiments can be used to enrich and accelerate the progress of accurate SARs in the development of drugs and therapeutics. The data handling and processing of affinity/kinetic and thermodynamic data will be applicable to other biophysical characterisation techniques.
Entry Requirements (not applicable to Visiting Students)
||Other requirements|| None
|Additional Costs|| 0
Course Delivery Information
|Academic year 2016/17, Not available to visiting students (SS1)
|Learning and Teaching activities (Further Info)
Lecture Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
|Assessment (Further Info)
|Additional Information (Assessment)
||In Course Assessment - 100%
An ICA consisting of a data analysis of an example dataset of affinity, kinetic and thermodynamic data and generation of report describing development of further experiments/considerations for development of the theoretical drug/therapeutic based on the SARs generated/supplied.
|No Exam Information
On completion of this course, the student will be able to:
- ┐ Demonstrate an understanding of the technologies that underpin SPR instruments and experiments.
- ┐ Demonstrate the ability to successfully generate and analyse an example dataset of affinity, kinetic and thermodynamic data.
- ┐ Discuss their results in a relation to the basic SARs of a model example protein:small molecule system.
- ┐ llustrate an understanding of the interplay between, affinity, stability, kinetics and thermodynamics in relation to protein:ligand interactions.
- ┐ Analyse SPR data sets and rationalise the effect of temperature on the kinetics and then extrapolate this to a ┐thermodynamic profile┐ of the protein:ligand interaction.
|Graduate Attributes and Skills
||Understanding of Surface plasmon resonance and its uses
|Course organiser||Dr Martin Wear
|Course secretary||Ms Andrea Nichol
Tel: (0131 6)50 8643
© Copyright 2016 The University of Edinburgh - 3 February 2017 3:25 am