Postgraduate Course: Practical Skills in Biochemistry A (PGBI11098)
|School||School of Biological Sciences
||College||College of Science and Engineering
|Credit level (Normal year taken)||SCQF Level 11 (Postgraduate)
||Availability||Not available to visiting students
|Summary||The programme provides thorough training and hands-on experience in fundamental practical skills required for employment as a biochemical scientist. The course will cover wet laboratory work, laboratory bookkeeping, health and safety, a small amount of bioinformatic analysis and numeracy.
Entry Requirements (not applicable to Visiting Students)
||Other requirements|| Pre-requisite: Undergraduate degree in either a biological or chemical science
Course Delivery Information
|Academic year 2018/19, Not available to visiting students (SS1)
|Learning and Teaching activities (Further Info)
Lecture Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
|Assessment (Further Info)
|Additional Information (Assessment)
||Three in-course assessments:
Practical skills and laboratory book are assessed weekly (20 %)
written report - results and analysis of data collected (own and whole class) in weeks 3 and 4 (20 %)
full experimental write-up of practicals in weeks 6-10 (60 %)
||Students will receive individual verbal feedback weekly on their skills and lab books during the laboratory sessions. Individual written feedback will be provided on each of the two written assessments within 15 working days of the submission deadline.
|No Exam Information
On completion of this course, the student will be able to:
- Prepare analytical quality solutions and dilution series ; determine concentrations of biological molecules by, and understand relative advantages of, a range of methods
- Design and execute a strategy for cloning of recombinant proteins, from primer design to the amplification, purification, restriction enzyme digest, ligation and transformation of DNA; through to the analysis of DNA sequencing data.
- Correctly operate and calibrate, or understand principles of calibration of, fundamental laboratory equipment.
- Analyse and manipulate experimental data, and present experiments and results in appropriate format.
- Understand the health and safety responsibilities required for a laboratory
|Practical Skills in Biomolecular Sciences by R. Reed et al, Pearson Education. |
Chapter on DNA cloning in general biochemistry textbooks, e.g.
Molecular Cell Biology by Lodish et al. W. H. Freeman.
Biochemistry by Berg et al. W. H. Freeman.
Molecular Biology of the Cell by Alberts et al, Garland Science.
For very detailed experimental protocols:
Molecular cloning: a laboratory manual by Sambrook et al. Cold Spring Harbor Laboratory Press.
|Graduate Attributes and Skills
||confidence in laboratory environment
appreciation of health and safety responsibilities
|Course organiser||Dr Janice Bramham
Tel: (0131 6)50 4786
|Course secretary||Ms Andrea Nichol
Tel: (0131 6)50 8643