Postgraduate Course: Quantitating drug binding (PGBI11038)
Course Outline
School | School of Biological Sciences |
College | College of Science and Engineering |
Credit level (Normal year taken) | SCQF Level 11 (Postgraduate) |
Availability | Not available to visiting students |
SCQF Credits | 10 |
ECTS Credits | 5 |
Summary | In four practical sessions, students in will determine the binding constant of an agonist to rat-brain muscarinic acetylcholine receptor using a filter assay to measure bound receptor. Further sessions will derive the binding constants and total number of receptors from the data, including use of non-linear least squares fit to the hyperbola describing binding. This analysis will be used to determine variances for the parameters fitted and will lead to a critical examination of the design of the experiment. Other techniques for determining binding constants will be considered, with an emphasis on the appropriate windows in which they can be applied and on potential artefacts and pitfalls. |
Course description |
Lecture Outlines
Week Title
1 Introduction
2 Setting the biological context
3 Practical - Day1
4 Practical - Day 2
5 Practical - Day 3
6 Data analysis
7 Practical Day 4
8 Fitting binding isotherms - practice
9 Fitting and evaluating different models for binding.
10 Formative Peer Assessment on Webfolios.
|
Entry Requirements (not applicable to Visiting Students)
Pre-requisites |
|
Co-requisites | |
Prohibited Combinations | |
Other requirements | None |
Course Delivery Information
|
Academic year 2019/20, Not available to visiting students (SS1)
|
Quota: 23 |
Course Start |
Semester 1 |
Timetable |
Timetable |
Learning and Teaching activities (Further Info) |
Total Hours:
100
(
Lecture Hours 6,
Supervised Practical/Workshop/Studio Hours 14,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
78 )
|
Assessment (Further Info) |
Written Exam
0 %,
Coursework
100 %,
Practical Exam
0 %
|
Additional Information (Assessment) |
Extended Practical report - 100 %
|
Feedback |
Not entered |
No Exam Information |
Learning Outcomes
On completion of this course, the student will be able to:
- acquire a deeper knowledge and critical appreciation of how binding constants for drug molecules to their targets are determined;
- find references and read into an academic subject given a starter reference;
- be competent to carry out the experimental manipulations to determine a binding constant of a drug to its target using a radioactive filter assay;
- understand how to derive a binding constant of a drug to its target and the stoichiometry;
- apply non-linear least squares methods to fit equations and to derive the variances in the parameters fitted.
|
Additional Information
Graduate Attributes and Skills |
Not entered |
Keywords | QDB |
Contacts
Course organiser | Dr Paul McLaughlin
Tel: (0131 6)50 7060
Email: paul.mclaughlin@ed.ac.uk |
Course secretary | Mrs Claire Black
Tel: (0131 6)50 8637
Email: Claire.Black@ed.ac.uk |
|
|