Postgraduate Course: Practical Skills in Biochemistry B (PGBI11099)
Course Outline
| School | School of Biological Sciences |
College | College of Science and Engineering |
| Credit level (Normal year taken) | SCQF Level 11 (Postgraduate) |
Availability | Not available to visiting students |
| SCQF Credits | 10 |
ECTS Credits | 5 |
| Summary | The programme provides thorough training and hands-on experience in enhanced practical skills required for employment as a biochemical scientist. The course will cover a range of techniques from recombinant protein overexpression and purification, followed by activity assays and biophysical characterisation. |
| Course description |
The course will be mostly taught in a dedicated practical laboratory with occasional use of a computer laboratory for data analysis.
Students will
- undertake a range of chromatographic techniques to purify and assess an enzyme
- design and implement a straightforward enzyme assay
- identify and implement several biochemical techniques to characterise the purified enzyme
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Course Delivery Information
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| Academic year 2020/21, Not available to visiting students (SS1)
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Quota: 19 |
| Course Start |
Semester 2 |
Timetable |
Timetable |
| Learning and Teaching activities (Further Info) |
Total Hours:
100
(
Supervised Practical/Workshop/Studio Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
68 )
|
| Assessment (Further Info) |
Written Exam
0 %,
Coursework
100 %,
Practical Exam
0 %
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| Additional Information (Assessment) |
Three in-course assessments:
Practical skills and laboratory books are assessed weekly (20 %)
Design of an experimental strategy for biophysical and activity characterisation of purified protein (30 %)
A report, in the form, of a paper, of all experiments performed (50 %)
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| Feedback |
Students will receive individual verbal feedback weekly on their skills and lab books during the laboratory sessions. Individual written feedback will be provided on each of the two written assessments within 15 working days of the submission deadline. |
| No Exam Information |
Learning Outcomes
On completion of this course, the student will be able to:
- Apply, or understand how to apply, a range of chromatographic techniques to purify a protein and then characterise it.
- Design and analyse a straightforward enzyme assay
- Identify the techniques required and design an appropriate strategy for the biophysical characterisation of a biomolecule; and apply or be able to explain the advantages and limitations of the methods identified.
- Describe how and why differences in experimental conditions, or available equipment, will influence the experimental strategy and outcome
- Understand the requirements for writing a publication-quality research paper
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Reading List
Read an occasional, relevant chapter, not necessarily for purchase.
Principles and Techniques of Biochemistry and "Molecular Biology" by A. Hoffman and S. Clokie, Cambridge University Press.
"Protein Purification Techniques: A Practical Approach" by S. Roe, Oxford University Press.
"Biophysical Techniques" by I.D. Campbell, Oxford University Press.
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Additional Information
| Graduate Attributes and Skills |
Confidence in laboratory environment
Report writing
Data management
Appreciation of health and safety responsibilities
Time management
Organisation
Group work
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| Keywords | BioChemB |
Contacts
| Course organiser | Dr Janice Bramham
Tel: (0131 6)50 4786
Email: janice.bramham@ed.ac.uk |
Course secretary | Ms Andrea Nichol
Tel: (0131 6)50 8643
Email: Andrea.Nichol@ed.ac.uk |
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