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DRPS : Course Catalogue : School of Chemistry : Chemistry

Postgraduate Course: Chromatography and separation science (CHEM10067)

Course Outline
SchoolSchool of Chemistry CollegeCollege of Science and Engineering
Credit level (Normal year taken)SCQF Level 11 (Postgraduate) AvailabilityNot available to visiting students
SCQF Credits10 ECTS Credits5
SummaryThis course aims to provide training in the principles and practice of chromatographic techniques.
The course material covers the fundamental principle of chromatography. In addition, the course details various chromatographic methods and how these are applied to a wide range of analytes. The course forms a part of the curriculum for any student enrolled on the PGT MSc degree course in Analytical Chemistry.
Course description The course consists of a blend of lectures, tutorials, workshops and hands-on experimental activities, which deal with the key concepts of chromatography and its application to real-world analytical challenges. The course aims to develop, the students ability to think critically and creatively and solve analytical problems. Students will be assessed on their performance in an exam and on their performance in hands-on activities. The course topics include:

- Theory of separation techniques
- Stationary phases (including ion exchange, ion pairing and chiral)
- Mobile phases
- Partition Coefficients, Retention Factors
- Separation and Resolution
- Theoretical Plate Model
- Thin layer chromatography (TLC) and flash chromatography
- High performance liquid chromatography (HPLC / UPLC / normal/reverse/chiral phase)
- Gas chromatography (system considerations and detection methods).
- Gel permeation chromatography
- Chromatographic Problem Solving - peak fronting, peak tailing, optimising column efficiency
- Related techniques (Supercritical Fluid Chromatography and Capillary Zone Electrophoresis)
- Gel electrophoresis principles
- Capillary electrophoresis principles
- Introduction to protein purification (affinity, Ion exchange, Size exclusion chromatography, SDS-PAGE)
Entry Requirements (not applicable to Visiting Students)
Pre-requisites Co-requisites
Prohibited Combinations Other requirements None
Course Delivery Information
Academic year 2022/23, Not available to visiting students (SS1) Quota:  None
Course Start Semester 1
Timetable Timetable
Learning and Teaching activities (Further Info) Total Hours: 100 ( Lecture Hours 12, Seminar/Tutorial Hours 18, Summative Assessment Hours 2, Revision Session Hours 2, Programme Level Learning and Teaching Hours 2, Directed Learning and Independent Learning Hours 64 )
Assessment (Further Info) Written Exam 50 %, Coursework 50 %, Practical Exam 0 %
Additional Information (Assessment) 50% coursework
50% Examination
Feedback Not entered
No Exam Information
Learning Outcomes
On completion of this course, the student will be able to:
  1. Discuss the principles and application of commonly used laboratory separation techniques such as gas chromatography, liquid chromatography and electrophoresis.
  2. Design chromatographic strategies for the separation/purification of a range of analytes, small organic molecules, peptides, proteins.
  3. Analyse samples on a range of common chromatographic systems.
Reading List
Additional Information
Graduate Attributes and Skills Understanding of the principles and applications of chromatography.
Learn which chromatographic methods are most suitable for the analysis of specific analytes.
Acquire practical skills in running common chromatographic separation experiments - HPLC, FPLC, gel electrophoresis.
KeywordsNot entered
Course organiserDr Annamaria Lilienkampf
Tel: (0131 6)50 4812
Course secretaryMs Zoe Burger
Tel: (0131 6)50 7546
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