Postgraduate Course: Chromatography and separation science (CHEM10067)
|School||School of Chemistry
||College||College of Science and Engineering
|Credit level (Normal year taken)||SCQF Level 11 (Postgraduate)
||Availability||Not available to visiting students
|Summary||This course aims to provide training in the principles and practice of chromatographic techniques.
The course material covers the fundamental principle of chromatography. In addition, the course details various chromatographic methods and how these are applied to a wide range of analytes. The course forms a part of the curriculum for any student enrolled on the PGT MSc degree course in Analytical Chemistry.
The course consists of a blend of lectures, tutorials, workshops and hands-on experimental activities, which deal with the key concepts of chromatography and its application to real-world analytical challenges. The course aims to develop, the students ability to think critically and creatively and solve analytical problems. Students will be assessed on their performance in an exam and on their performance in hands-on activities. The course topics include:
- Theory of separation techniques
- Stationary phases (including ion exchange, ion pairing and chiral)
- Mobile phases
- Partition Coefficients, Retention Factors
- Separation and Resolution
- Theoretical Plate Model
- Thin layer chromatography (TLC) and flash chromatography
- High performance liquid chromatography (HPLC / UPLC / normal/reverse/chiral phase)
- Gas chromatography (system considerations and detection methods).
- Gel permeation chromatography
- Chromatographic Problem Solving - peak fronting, peak tailing, optimising column efficiency
- Related techniques (Supercritical Fluid Chromatography and Capillary Zone Electrophoresis)
- Gel electrophoresis principles
- Capillary electrophoresis principles
- Introduction to protein purification (affinity, Ion exchange, Size exclusion chromatography, SDS-PAGE)
Entry Requirements (not applicable to Visiting Students)
||Other requirements|| None
Course Delivery Information
|Academic year 2022/23, Not available to visiting students (SS1)
|Learning and Teaching activities (Further Info)
Lecture Hours 12,
Seminar/Tutorial Hours 18,
Summative Assessment Hours 2,
Revision Session Hours 2,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
|Assessment (Further Info)
|Additional Information (Assessment)
|No Exam Information
On completion of this course, the student will be able to:
- Discuss the principles and application of commonly used laboratory separation techniques such as gas chromatography, liquid chromatography and electrophoresis.
- Design chromatographic strategies for the separation/purification of a range of analytes, small organic molecules, peptides, proteins.
- Analyse samples on a range of common chromatographic systems.
|Graduate Attributes and Skills
||Understanding of the principles and applications of chromatography.
Learn which chromatographic methods are most suitable for the analysis of specific analytes.
Acquire practical skills in running common chromatographic separation experiments - HPLC, FPLC, gel electrophoresis.
|Course organiser||Dr Annamaria Lilienkampf
Tel: (0131 6)50 4812
|Course secretary||Ms Zoe Burger
Tel: (0131 6)50 7546